essay like date January 2011 A monolithic HPLC column is a special type of column used in HPLC with porous channels rather than beads. High performance liquid chromatography HPLC is the third most widely used laboratory instrument, surpassed only by analytical balance s and pH meter s. Advances in HPLC are evolutionary, not revolutionary, moving forward incrementally over long periods of time. HPLC column technologies are no exception. HPLC columns are consumable drop ins for the HPLC instrument, yet they are arguably the most important part. It is the chemistry and structure of the HPLC column that allows for efficient separations. In the long history of HPLC, particulate packed columns have ... in the HPLC columns, as well as the history and the industries in which they play a role, will be discussed ... of separation in HPLC rely on a mobile phase water, organic solvent s, etc. being passed through ... competitively against traditionally packed columns. Historically, HPLC columns have been packed ... backpressures present a significant limitation to the chromatographer. Modern HPLC systems are generally ... procedure ref Porous monoliths the newest generation of stationary phases for HPLC and related ... to withstand the rigors of HPLC. In this decade, affinity chromatography was invented, an ultra ... importantly, the modern HPLC was born. Csaba Horvath led the development of modern HPLC by piecing ... commercially available HPLC as a Nucleic Acid Analyzer. The following year, the first international symposia on HPLC was held, and Kirkland at DuPont was able to functionalize controlled porosity ... in this field. New technologies in HPLC generally start in small research labs in academia or government ... selecting an HPLC column supplier, column lifetime was second only to column to column reproducibility ... modern HPLC was designed and marketed as a nucleic acid analyzer. Columns throughout the 1970s were ... analyses in the 1980s, when computerized controls were integrated into HPLC equipment. Higher degrees ... more details
A sample injector is a device used in conjunction with injecting Sample material samples into high performance liquid chromatography HPLC or similar chromatography apparati. References reflist External links http weather.nmsu.edu teaching Material soil698 Student Material hplchp1090 hplcinj.htm HPLC injector category chromatography Science stub ... more details
Shodex is a brand of High performance liquid chromatography HPLC columns and is best known for innovative size exclusion chromatography and sugar analysis columns. In addition, Shodex has introduced highly effective Restricted Access Material RAM columns for both polar and non polar small molecule analysis. The new solid sphere ionic exchange columns for ultra fast protein analysis can be used in conventional HPLC or UHPLC instruments. Recently, an ultra high temperature reversed phased column operating up to 150 Degrees Celsius providing enhanced control in selectivity was introduced. Shodex HPLC Columns are made in Japan by Showa Denko , one of the largest Japanese chemical companies. They produce more than 800 different columns, most packed with polymer based packing material, and have been doing so since 1973. Products include standard analysis columns, semi micro and micro columns, and preparative columns. They also manufacture HPLC instruments such as refractive index RI detectors and degassing devices. Shodex is distributed in the US by Showa Denko America as well as by a range of analytical products makers and distributors. External links http www.shodex.com Shodex Worldwide General website http www.shodex.net Shodex North and Latin America website http www.shodex.de Shodex Europe website Category Chromatography ... more details
refer Tagging of Pacific Predators , a project by Census of Marine Life The OpenMS Proteomics Pipeline , a set of computational tools to solve HPLC MS data pipeline analysis problem Topp may refer to a List of Norwegian magazines Norwegian magazine See also TOP disambiguation Topps disambiguation ... more details
Solvophobic theory solvophobicity attempts to explain interactions between Chemical polarity polar solvent s and non polar solute s. The molecular structure of the solvent molecule is driven by hydrogen bonding between similarly structured solvent molecules but is perturbed by non polar solute molecules. ref Satinder Ahuja, Selectivity and detectability optimizations in HPLC , ISBN 0471626457 ref When applied to liquid chromatography LC , solvophobic theory attributes the retention of solutes on the stationary phase to the active rejection of solute molecules by the solvent. However, in practice attractive forces between the solvent and the stationary phase play a role in driving the retention of solvents in LC systems. ref Satinder Ahuja, Selectivity and detectability optimizations in HPLC , ISBN 0471626457 ref References Reflist Category Solutions Category Chromatography Category Separation processes physical chemistry stub ... more details
LSSR may refer to The Lake Superior Scottish Regiment , a Canadian Infantry regiment Latvian Soviet Socialist Republic , now Latvia Lithuanian Soviet Socialist Republic , now Lithuania Lunar Surface Sample Return Linear Solvent Strength Relationship , a theory used in high performance liquid chromatography HPLC disambig de LSSR eo LSSR ... more details
Summary Information Description This is an Agilent 1200 HPLC with automatic sample carriage, quaternary pump, multiple wavelength detector and no column fitted, connected via ethernet to a PC. Source I took this photo myself. Date 11 8 2008 Author User Kommando Kommando User talk Kommando talk other versions Licensing self cc by sa 3.0 GFDL migration redundant ... more details
Unreferenced date July 2007 otheruses Monolithic HPLC column File Colonne pompee 2.JPG thumb 300px Pompey s Pillar column Pompey s Pillar , a Roman Empire Roman monolithic column in Alexandria , Egypt A monolithic column is a column of which the shaft is made from one single piece of stone instead of different sections. They are sometimes called single piece columns and were most likely used on ancient temples. Category Columns and entablature Category Monoliths element arch stub monolithic pillars are one of the best specimens of mauryan architecture. ... more details
SVG available Bi and ter metallocenes containing rhodocenyl groups.svg Diagram of the hexafluorophosphate salts of rhodocenylferrocene, 1,1 dirhodocenylferrocene, and 1 cobaltocenyl l rhodocenylferrocene. The image is adapted from cite journal title Synthesis and Preparative HPLC Separation of Heteronuclear Oligometallocenes. Isolation of Cations of Rhodocenylferrocene, 1,1 Dirhodocenylferrocene, and 1 Cobaltocenyl 1 rhodocenylferrocene author1 Andre,M. author2 Schottenberger, H. author3 Tessadri, R. author4 Ingram, G. author5 Jaitner, P. author6 Schwarzhans, K. E. journal Chromatographia volume 30 issue 9&ndash 10 pages 543&ndash 545 doi 10.1007 BF02269802 However, as simple chemical structures they are not copyrightable. PD chem ... more details
In chemistry , sparging , also known as gas flushing in metallurgy , is a technique which involves bubbling a chemically inert gas, such as nitrogen , argon , or helium , through a liquid. This can be used to degasification remove dissolved gases e.g. oxygen from the liquid. Solvents used in high performance liquid chromatography HPLC are often sparged with helium gas. ref cite journal journal Today s Chemist at Work publisher American Chemical Society volume 9 issue 8 pages 28 32 year 2000 author M. W. Dong title Precision in HPLC. Mastering the art of HPLC id ISSN 1062 094X ref In chemical engineering , sparging can also be a method to remove low boiling liquids from a solution. The low boiling components tend to evaporate more rapidly, hence they may be removed from the bulk solution containing higher boiling components. It is an alternative to distillation , and it does not require heat. This technique is also used in environmental chemistry to extract the oil contaminants from subsoil water and the ground. In metallurgy, this process is used to remove dissolved gases from the melt prior to the material being processed. ref Cite book last Degarmo first E. Paul last2 Black first2 J T. last3 Kohser first3 Ronald A. title Materials and Processes in Manufacturing publisher Wiley page 284 year 2003 edition 9th isbn 0 471 65653 4 ref For example, before casting aluminium alloys , argon gas bubbles can be injected in liquid aluminium using a rotary degaser. The resulting argon bubbles will go up to the surface bringing with it a quantity of dissolved hydrogen gas. This degasing treatment reduces the occurrence of hydrogen gas porosity . References reflist External links http books.google.com books?id KCUjfz ILSEC&pg PA86&lpg PA86&dq gas flushing melt&source bl&ots gGwhuIlMoN&sig Pk5lJ3h 7D M3tHI6nbvRFfxyQw&hl en&sa X&oi book result&resnum 5&ct result PPA86,M1 Gas flushing of copper Category Laboratory techniques Category Metallurgy fr Barbotage ru uk ... more details
Infobox software name Agilent ChemStation logo screenshot caption developer latest release version latest release date release date 2009 3 24 operating system Windows genre Liquid chromatography software license Proprietary software proprietary website http www.chem.agilent.com en US products software datasystems chemstation pages default.aspx ChemStation on Agilent website Agilent ChemStation is a software package to control Agilent liquid chromatography and gas chromatography systems such as the 1050, 1100 and 1200 Series HPLC system. It is an evolution of the Hewlett Packard ChemStation System. Two versions are available one online in connection with the modules of the HPLC chain is designed to control instruments and run experiments, and the other offline , without a connection with the HPLC chain, is designed to analyze data. ChemStation is structured around a number of register s dn . Two of the more important registers are CHROMREG and CHROMRES, the chromatographic data registers. ChemStation has a command line interpreter and can run Macro computer science macro s. Those macros are files grouping a set of commands. These files possess a .mac extension. ChemStation can import analysis lists and export result files in XML by adding new lines to the ChemStation.ini configuration file. This is a feature to implement the connection with a Laboratory information management system LIMS . External links http www.chem.agilent.com en US products software datasystems chemstation pages default.aspx DEFAULTSORT Agilent Chemstation Category Chromatography software Category Laboratory Information Management System science software stub ... more details
Infobox chemical analysis name High performance liquid chromatography image hplc.JPG caption An HPLC ... column and an apparatus for measuring the absorbance. acronym HPLC classification Chromatography ... liquid chromatography hyphenated Liquid chromatography mass spectrometry File HPLC apparatus.svg thumb HPLC apparatus. Cleanup jargon date June 2010 High performance liquid chromatography or high pressure liquid chromatography , HPLC is a chromatographic technique that can separate a mixture of compounds ... components of the mixture. HPLC typically utilizes different types of stationary phases ..., and higher mobile phase pressures. With HPLC, a pump rather than gravity provides the higher pressure .... A further refinement of HPLC is to vary the mobile phase composition during the analysis gradient ... the HPLC method which gives the best peak separation. Types Partition chromatography File Agilent1200HPLC.jpg thumb A modern self contained HPLC. Partition chromatography was the first kind of chromatography ... phase and the eluent. Known as Hydrophilic Interaction Chromatography HILIC in HPLC, this method ... phase chemistry stationary phase and a non polar, water miscible , mobile phase. Partition HPLC has ... Also known as normal phase HPLC NP HPLC , or adsorption chromatography, this method separates analytes ... kinds of HPLC that chemists developed. NP HPLC uses a polar stationary phase and a non polar, non ... . Partition and NP HPLC fell out of favor in the 1970s with the development of reversed phase HPLC ... chromatography RPC File Hplc perfume chromatogram.png thumb 300px A chromatogram of complex mixture perfume water obtained by reversed phase HPLC details Reversed phase chromatography Reversed phase HPLC RP HPLC or RPC has a non polar stationary phase and an aqueous, moderately polar mobile phase ... incorrectly referred to as HPLC without further specification. The pharmaceutical industry regularly ... long, as it can corrode the metal parts of the HPLC equipment. RP HPLC columns should be flushed ... more details
, SFC is as simple and robust as high performance liquid chromatography HPLC but fraction collection ... volume of polar co solvent. Similar to an HPLC system, SFC can use a variety of detection methods including Ultraviolet UV VIS, mass spectrometry , Flame ionization detector FID unlike HPLC and evaporative ... more details
italic title taxobox name Eremanthus regnum Plantae unranked divisio Angiosperms unranked classis Eudicots unranked ordo Asterids ordo Asterales familia Asteraceae genus Eremanthus genus authority Less. subdivision ranks Species subdivision 22 See text. Eremanthus Less is a genus of plant s belonging to the Asteraceae family with 22 recorded species . All plants are native of Cerrado in Brazil . Eremanthus species are used as food plants by the larva e of some Lepidoptera species including Dalcera Dalcera abrasa which has bee recorded on E. glomerulatus . References Sakamoto, H. T., et al. 2005 http www.scielo.br scielo.php?script sci arttext&pid S0103 50532005000800016&lng en&nrm iso Quantitative HPLC analysis of sesquiterpene lactones and determination of chemotypes in Eremanthus seidelii MacLeish & Schumacher Asteraceae . J. Braz. Chem. Soc. 16 6b 1396 1401 accessed 27 March 2006 Category Asteraceae genera asteraceae stub pt Eremanthus ... more details
SVG available Synthesis of 1 cobaltocenyl 1 rhodocenylferrocene cation.svg Synthesis of the 1 cobaltocenyl 1 rhodocenylferrocene cation based on the method described in cite journal title Synthesis and Preparative HPLC Separation of Heteronuclear Oligometallocenes. Isolation of Cations of Rhodocenylferrocene, 1,1 Dirhodocenylferrocene, and 1 Cobaltocenyl 1 rhodocenylferrocene author1 Andre,M. author2 Schottenberger, H. author3 Tessadri, R. author4 Ingram, G. author5 Jaitner, P. author6 Schwarzhans, K. E. journal Chromatographia volume 30 issue 9&ndash 10 pages 543&ndash 545 doi 10.1007 BF02269802 cite journal title Termetallocenes author1 Jaitner, P. author2 Schottenberger, H. author3 Gamper, S. author4 Obendorf, D. journal J. Organomet. Chem. volume 475 issue 1&ndash 2 year 1994 pages 113&ndash 120 doi 10.1016 0022 328X 94 84013 X Simple chemical structures so content is non copyrightable. PD chem ... more details
A list of chemical analysis methods with acronyms. CompactTOC8 name no center no top yes seealso yes A Atomic absorption spectroscopy AAS Atomic emission spectroscopy AES Atomic fluorescence spectroscopy AFS APXS Alpha particle X ray spectrometer APXS C Capillary electrophoresis CE Chromatography Colorimetry Computed tomography Cyclic Voltammetry CV D Differential scanning calorimetry DSC E Electron paramagnetic resonance EPR also called Electron spin resonance ESR Energy Dispersive Spectroscopy EDS EDX F Field flow fractionation FFF Flow injection analysis FIA Fourier transform spectroscopy FTIR G Gas chromatography GC Gas chromatography mass spectrometry GC MS Gas chromatography IR spectroscopy GC IR Gel permeation chromatography IR spectroscopy GPC IR H High performance liquid chromatography HPLC High performance liquid chromatography IR spectroscopy HPLC IR I Microprobe Ion Microprobe IM Inductively coupled plasma ICP Ion selective electrode ISE e.g. determination of pH L Laser induced breakdown spectroscopy LIBS Liquid chromatography IR spectroscopy LC IR Liquid chromatography mass spectrometry LC MS M Mass spectrometry MS Mossbauer spectroscopy N Neutron activation analysis Nuclear magnetic resonance NMR P PIXE Particle induced X ray emission spectroscopy PIXE Pyrolysis gas chromatography mass spectrometry PY GC MS R Raman spectroscopy Refractive index Resonance enhanced multiphoton ionization REMPI S Scanning electron microscope SEM Scanning X ray microscope SXM also called Scanning transmission X ray Microscope STXM T Transmission electron microscopy TEM X X ray diffraction XRD X ray fluorescence X ray fluorescence spectroscopy XRF X ray microscopy XRM See also Analytical chemistry List of materials analysis methods Category Analytical chemistry hu M szeres analitikai m dszerek no Liste over kjemiske analysemetoder ... more details
Infobox Company company name Dionex Corporation company logo company type Corporation company slogan foundation location Flag USA Sunnyvale, California , United States of America USA key people Frank Witney, Ph.D. President and Chief Executive Officer ref http investor.dionex.com releasedetail.cfm?ReleaseID 377026 ref industry products chromatography revenue num employees homepage http www.dionex.com en us index.html http www.dionex.com microsite http dionex uhplc.com http www.dionex uhplc.com Dionex Corporation Nasdaq DNEX is an American company based in Sunnyvale, California . It develops, manufactures, sells, and services chromatography , ion chromatography and Liquid liquid extraction extraction systems for separating, isolating, and identifying the components of chemical mixtures. Dionex develops the Chromeleon Chromeleon brand of chromatography software . The primary function of Chromeleon is to control and obtain data from Dionex analytical instruments although the software supports instruments from other vendors. Chromeleon 7 is the latest edition of the software. The latest technology made by Dionex is the Rapid Separation LC RSLC and the polymeric HPLC columns, a type of monolithic HPLC column . Unlike the inorganic silica columns, the polymer monoliths are made of an organic polymer base. Dionex, traditionally known for its ion chromatography capabilities, has led this side of the field. Dionex first acquired a license for the polymeric monolith technology in the 1990s. Thermo Fisher Scientific announced its acquisition of Dionex in December 2010. References reflist External links http www.nasdaqomx.com whatwedo servicesforcompanies usmarket institutionalinvestorcenter companypresentations companypresentationspage ?contentId 20616 Dionex on nasdqomx.com Category Companies based in California US company stub chemistry stub pl Dionex Corporation ... more details
and acid pH has been used for many years, cation exchange HPLC 3 4 5 6 7 8 9 10 is emerging as the method ... . The use of HPLC streamlines the recommended preliminary and follow up tests for the identification ... of cases. The elements of one approach include a CBC, Hb H test, ferritin, HPLC for Hb A2 and F ... scanning of electrophoretic gels is poor, especially when compared with HPLC techniques 8 . A recent ... was 14.6 at a mean Hb A2 concentration of 3.21 , and for HPLC, the CV was 4.3 at a mean Hb A2 of 3.47 ... and HPLC to identify and quantify Hb. IEF IEF is an electrophoretic technique with excellent resolution ... electrophoresis sensitivity of detection with the automated sampling and data acquisition of HPLC ... D Punjab. Capillary Zone Electrophoresis hplc Cation exchange HPLC is emerging as the method of choice ... cation exchange HPLC instrument that has been used to quantify Hb A2, Hb F, Hb A, Hb S, and Hb ... methods. HPLC is not without intrinsic interpretive problems. Hbs may co elute or may elute ... A2 using cation exchange HPLC is complicated in individuals with Hb S 20 21 because the Hb A2 is falsely ... methods and are higher than those obtained by HPLC techniques. Immunodiffusion techniques ... HPLC has been used as an enhancement for electrospray ionization spectroscopy in the characterization of Hb Rambam 69 GlyAsp 24 . This represents an additional novel use of HPLC in Hb variant diagnosis. Liquid chromatography mass spectroscopy has been used to characterize other variant Hbs 25 . HPLC ... HPLC and low results using a latex immunoagglutination method. Hb Sherwood Forest 29 , Rambam 24 , and Raleigh 27 produce falsely increased Hb A1C results when measured by cation HPLC. dna analysis ... more details
. TLC methods and HPLC are commonly used. HPLC alone is not sufficient as it may often yield false positive results. Today HPLC MSMS analysis is used to quantify and confirm the presence of zearalenone ... more details
Infobox Company company name Phenomenex, Inc. company logo Image Phenomenex logo.JPG 200px company type Private company foundation 1982 location Torrance, California Torrance, CA, USA key people Fasha Mahjoor , Entrepreneur Founder and Chief executive officer CEO area served industry Manufacturing products High performance liquid chromatography HPLC , Gas liquid chromatography GC and Solid phase extraction SPE consumables revenue operating income net income num employees parent subsid Australia , Austria , Belgium , Denmark , Finland , France , Germany , Italy , Luxembourg , Netherlands , New Zealand , Norway , and UK homepage http www.phenomenex.com http www.phenomenex.com footnotes Phenomenex, Inc. is a consumables company currently based in Torrance, California Torrance , California , USA . Phenomenex is dedicated to the manufacture of High performance liquid chromatography HPLC , Gas liquid chromatography GC and Solid phase extraction SPE columns, as well as other consumables applying to column chromatography techniques. As of 2005, Phenomenex held close to a 15 market share worldwide in HPLC columns and more than 20 just in the USA. ref http www.phenomenex.com company display.aspx?id 6813&ekmensel 8 submenu 0 link 4 Phenomenex Market Share Bot generated title ref Phenomenex has subsidiaries in Australia, Austria, Belgium, Denmark, Finland, France, Germany, Italy, Luxembourg, Netherlands, New Zealand, Norway, and the UK. Products & Services Liquid Chromatography Columns Gas Chromatography Capillary Columns Solid Phase Extraction Tubes & 96 Well Plates Sample Cleanup and Preparation Devices Oligonucleotide Purification Products Amino Acid Analysis Kits Bulk Chromatography Materials for Purification Chromatography Accessories and Liquid Handling Devices Filtration Devices Training Software and Reference Materials Technical and Method Development Services Application Support from Sample Preparation to Final Analysis Markets Served Academic Environmental Food and Be ... more details
DAD is an abbreviation that may refer to D A D , a Danish rock band formerly known as Disneyland After Dark Diode array detector , a type of detector in HPLC Da Nang International Airport IATA airport code Die Another Day , a 2002 motion picture starring Pierce Brosnan Digital Audio Disc Disinhibited attachment disorder , a psychological disorder in the ICD 10 IPv6 address Duplicate address detection Duplicate Address Detection , a component of the IPv6 electronic communications protocol, part of the Neighbor Discovery Protocol NDP Designated Advisor for Disclosure , an investment banker or a securities attorney knowledgeable in U.S. securities laws and OTCQX compliance rules Dark Armed Dragon, a very powerful and sought after card from the Yu Gi Oh trading card game Diffuse Alveolar Damage , a histological pattern of lung disease seen in Acute Respiratory Distress Syndrome and Acute Interstitial Pneumonia See also Dad disambiguation disambig de DAD eo DAD fr DAD it DAD ja DAD ... more details
chromatography or HPLC, a technique which became a major field of study and in which he remained ... the framework of the solvophobic theory. As HPLC and RPLC became the preeminent techniques associated ... more details
Primarysources article date September 2007 Infobox Company company name Presearch. company logo Image Presearch.JPG 148px Presearch company type Scientific foundation 1991 location Basingstoke, UK key people industry Scientific instrument s products Scientific instrument s revenue operating income net income num employees 20 parent subsid homepage http www.presearch.co.uk www.presearch.co.uk footnotes Presearch is a scientific instrument manufacturer. It was founded in 1991 with the aim of providing elegant solutions to emerging issues in High performance liquid chromatography HPLC and related techniques. Presearch is now the distributor for over 21 scientific manufacturers. In June 2005 Presearch was acquired by the Medical & Scientific division of United Drug plc, Irelands largest healthcare services provider. ref cite web title 30th June 2005 United Drug Acquires UK Distributor. Press release url http www.united drug.ie news presearch.asp ref References Reflist External links http www.Presearch.co.uk Presearch http www.united drug.ie medical.asp United Drug Category Chromatography UK company stub ... more details
Image Windmill operated water pump.jpg thumb 300px Reciprocating pump attached to a Windmill on a farm. A reciprocating pump is a positive plunger pump . It is often used where relatively small quantity of liquid is to be handled and where delivery pressure is quite large. Reciprocating pumps are used in High performance liquid chromatography High performance liquid chromatography HPLC . Examples Some examples of reciprocating pumps include br Windmill water pump br Hand pump br Axial piston pump br Radial piston pump See also Pump References http books.google.com books?id yU5TyJrOMF8C&pg PT498&lpg PT498&dq 22The basic principle of a reciprocating pump is that a solid will displace an 22 wikipedia&source bl&ots SAK24x Cu8&sig J2uCnREaaHPiRB1VYcEJEd6Dupc&hl en&ei 9H wSZ6TLIigM eQ3bEP&sa X&oi book result&ct result&resnum 1 Pump Handbook third edition EDITED BY Igor J. Karassik Joseph P. Messina Paul Cooper Charles C. Healdeald http www.maritime.org fleetsub hydr chap1.htm Maritime.org http www.roymech.co.uk Related Pumps Reciprocating 20Pumps.html Roymech.co.uk Category Pumps Tech stub ru uk zh ... more details
orphan date December 2009 The Purnell equation is an equation used in analytical chemistry to calculate the resolution R sub s sub between two peaks in a Chromatogram Chromatography terms chromatogram ref Miller, J. M., Chromatography Concepts and Contrasts , second edition, John Wiley & Sons, Inc., Hoboken, NJ, USA, 2005. ref ref Dean, J. R. et al., Practical Skills in Chemistry , first edition, Pearson Education Ltd., Harlow, UK, 2002, p. 205 224. ref ref http www.cristinalegido quigley.co.uk files LECTURE 202, 20FUNDAMENTALS 20HPLC.pdf C. Legido Quigley, HPLC theory ref math R s frac sqrt N 2 4 left frac alpha 1 alpha right left frac k 2 1 k 2 right math where R sub s sub is the resolution between the two peaks N sub 2 sub is the plate number of the second peak is the separation factor between the two peaks k sub 2 sub is the retention factor of the second peak. The higher the resolution, the better the separation. References reflist Category Chromatography Category Equations analytical chem stub ... more details
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