Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry analysis.^[1][2] The name is derived from the similar approach to DNA seqencing.^[3] Proteins are typically ionized by electrospray ionization and trapped in a Fourier transform ion cyclotron resonance (Penning trap)^[4] or quadrupole ion trap (Paul trap) mass spectrometer. Fragmentation for tandem mass spectrometry is accomplished by electron capture dissociation or electron transfer dissociation.

See also

• Protein mass spectrometry
• Bottom-up proteomics
• Shotgun proteomics

References

Bibliography