In biology, a subculture is either: a microbiological culture made by transferring microorganisms from a previous culture to a fresh growth medium, a method used to prolong the life of a particular strain of microorganism where there is a tendency of degeneration in older cultures; or a fresh culture made with material obtained from a previous one.

In plant tissue culture somaclonal variation can be reduced by decreasing the numbers of subcultures used in protocols.

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