Shotgun proteomics is a method of identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.^[1][2][3] The name is derived from shotgun sequencing of DNA which is itself named by analogy with the rapidly-expanding, quasi-random firing pattern of a shotgun. In shotgun proteomics, the proteins in the mixture are digested and the resulting peptides are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides.

See also

• Protein mass spectrometry
• Bottom-up proteomics
• Top-down proteomics
• Mass spectrometry software

References

Bibliography