The SAM riboswitch (also known as the S-box leader and now also called the SAM-I riboswitch) is found upstream of a number of genes which code for proteins involved in methionine or cysteine biosynthesis in Gram-positive bacteria. Two SAM riboswitches in Bacillus subtilis that were experimentally studied act at the level of transcription termination control. The predicted secondary structure consists of a complex stem-loop region followed by a single stem-loop terminator region. An alternative and mutually exclusive form involves bases in the 3' segment of helix 1 with those in the 5' region of helix 5 to form a structure termed the anti-terminator form.^[1][2][3] However, many SAM riboswitches are likely to regulate gene expression at the level of translation.

Structure organization

A 3D representation of the SAM riboswitch

The structure of the SAM riboswitch has been determined with X-ray crystallography. ^[4] The SAM riboswitch is organized around a four way junction, with two sets of coaxially stacked helices arranged side-by-side. These stacks are held together by a pseudoknot formed between the loop on the end of stem P2 and the J3/4 joining region. The formation of the pseudoknot is facillitated by a protein-independent kink-turn which induced a 100 ^o bend into P2. Both the kink-turn and the pseudoknot are critical to the establishment of the global fold and productive binding. The binding pocket is split between conserved, tandem AU pairs in stem P1 and the conserved asymmetric bulge in stem P3. The adenosyl and methionine main-chain moieties of S-Adenosyl methionine (SAM) are recognized through hydrogen-bonding into the bulge in P3. The methyl group is recognized indirectly through the charged sulfur, which forms an electrostatic interaction with the negative surface potential in the minor groove of P1. This negative surface is created by the tandem AU pairs.

Two classes of SAM-binding riboswitches are known that have entirely different structures to SAM-I riboswitches, these are SAM-II riboswitches and SAM-III riboswitches. A fourth type, SAM-IV riboswitches, appears to share a similar ligand-binding site with that of SAM-I riboswitches, but in the context of a distinct scaffold.

References

External links

• PDB entry for the SAM riboswitch tertiary structure

---

Source: Wikipedia | The above article is available under the GNU FDL. | Edit this article