Mule train can refer to:

• A connected line of mules, usually carrying cargo or riders.
  Mules hauling a 22,000lb boiler. Mule trains have been used as working (as opposed to tourist attractions, see Grand Canyon) portions of transportation links as recently as 2005 by the WFP http://www.wfp.org/english/?ModuleID=137&Key=1901.
  See also Twenty mule team.
• The song Mule Train
• Project Mule Train, which used C-123 aircraft, provided tactical airlift support for ground troops in South Vietnam, from December 11, 1961 to December 8, 1962.
• In the Korean War, the 5th Motor Transport Squadron was known as The Mule Train.
• A type of logging truck.
• A protest, lead by Martin Luther King, Jr. and Ralph David Abernathy, where protestors rode in covered wagons pulled by mules from Marks, Mississippi to Washington, D.C.
• The largest money laundering "sting" operation targeting a check cashing business in U.S. history was Operation Mule Train (July 1, 1998), initiated by the Los Angeles office of the FBI and the LAPD.