Deoxyribonuclease II, or Acid DNase, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. As the name implies, it is more effective at acid pHs.

There are several known DNases II, including:

• DNase II alpha (usually just called DNase II)
• DNase II beta (also called DLAD, or DNase II-Like Acid DNase)

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