Bio may refer to:

• bio, a prefix meaning life.
• Biographies
• Biology, the branch of science dealing with the study of life
• Biochemistry, the branch of Chemistry dealing with Metabolic reactions
• Bio (graffiti artist), a graffiti artist
• Bio., an Australian Pay-TV Channel
• Bio, Lot, a commune of the Lot département, in southwestern France
• Internet slang for "Biology break", also known as going to the bathroom. Typically someone will type "brb bio", meaning "be right back, taking a bathroom break"
• Bio is a set of Black Magic spells that causes Poison in Final Fantasy
• BIO is an abstraction library used by OpenSSL to handle communication of various kinds, including files and sockets, both secure and not
• Choudenshi Bioman, a Japanese television series.

BIO may stand for:

• Binocular Indirect Opthalmoscope, a medical instrument used to examine the interior of the eye
• Biotechnology Industry Organization
• Bilbao Airport (IATA airport)
• Bring it On, a film
• Break it Off, a single.
• British Informatics Olympiad, an annual programming competition in Great Britain.
• By Invitation Only
• Bedford Institute of Oceanography

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