In enzymology, an acetylputrescine deacetylase () is an enzyme that catalyzes the chemical reaction

N-acetylputrescine + H₂O \rightleftharpoons acetate + putrescine

Thus, the two substrates of this enzyme are N-acetylputrescine and H₂O, whereas its two products are acetate and putrescine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acetylputrescine acetylhydrolase. This enzyme participates in urea cycle and metabolism of amino groups.

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