AFI may refer to:

• AFI (band), an American rock band (abbreviated from "A Fire Inside")
• Agencia Federal de Investigación, a Mexican agency intended to fight corruption and organized crime
• Air Force Instructions, policies, directives, and guidelines for use by members of the United States Air Force
• American Football Israel, a nonprofit sports organization
• Amniotic Fluid Index, a measure of the amount of amniotic fluid, which is an index for fetal wellbeing
• Aquarium Fish International, a North American monthly magazine
• Armed Forces of India, the military organization responsible for the security and defence of India

Film institutes

• American Film Institute, an independent non-profit organization
• Australian Film Institute, an organisation that promotes Australian film and television

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